WebConfocal immunofluorescent analysis of THP-1 cells, untransfected (left) or transfected with Poly (I:C) (2.5 μg/ml, 6 hr; right), using Phospho-IRF-3 (Ser386) (E7J8G) XP ® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) #8584 (red). Blue = Hoechst 33342 #4082 (fluorescent DNA dye). Show More WebThough structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).
GAS-STING signaling plays an essential pathogenetic role in …
WebMar 3, 2024 · The interplay of VHL, phospho-TBK1, and p62 should also be further assessed in patient ccRCC tumors, as the relationship seems more complex than that found in cell line models, as suggested by the ambiguous results from the patient tissue microarrays presented in the study. Most importantly, the clinical role of TBK1 inhibitors in the ... WebAMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to … green country free clinic
Evaluating TBK1 as a Therapeutic Target in Cancers with …
WebApr 7, 2024 · Thirty minutes after AO, TBK1 activation at Ser 172 also became apparent and evidence of Parkin substrate (CISD1) ubiquitylation and LC3 maturation was also observed ( Fig. 2B ). At 60 min after AO, PINK1-phosphorylation of ubiquitin Ser 65 was increasingly apparent and continued to increase at later time points. WebMycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, … WebApr 8, 2024 · Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation Syntaxin 17 (Stx17) has been implicated in autophagosome-lysosome fusion. Here, we report that Stx17 functions in assembly of protein complexes during autophagy initiation. flow waterjet machine