Phgwfs7.0

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Websubsequent analysis Running the decideTests function using the global method from COMM 2024 at Simon Fraser University WebVIB-UGent Center for Plant Systems Biology. Gateway Vectors. Collection. pKGWFS7.

miRNAs control HAM1 functions at the single-cell-layer …

WebFeb 1, 2016 · genomic DNA and the binary vector pHGWFS7.0_RD29A (positive control) were completely digested with BamH1 and the GUS gene was used as probe. Transgenic lines (P-1 and P-2) s howed one band. Web... pHGWFS7 for promoter-gus constructs ( Fig. 1) : This vector with binary pPZP200 backbone ( Hajdukiewicz et al. 1994) contains CmR-ccdB selection flanked by attR1 and … phenom ventures

A Medicago truncatula lncRNA MtCIR1 negatively regulates

WebMar 24, 2024 · We prepared genomic fragments (1) from 4.2 kbp upstream of the translation start site of HAM1 (AT2G45160), which contains the promoter region, to the TS of miR171a; (2) from the TS to the 3′-UTR end; and (3) from the linearized vector (from pHGWFS7.0) by PCR using primer nos. 7 and 8, 9 and 10, and 11 and 12, respectively (see Supplemental ... WebApr 2, 2010 · Welcome to the Rausher Lab protocol library. Entries which are Wiki pages appear as links; entries which are downloadable files appear with green arrows after their names. WebJun 17, 2024 · The letters (a, b, c) indicate statistically significantly different values (P < 0.05), determined using Tukey’s HSD test (b is significantly different from a, c is significantly different from b). ... (Invitrogen) recombination, then … phenom united 3ssb

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Plant Tissue Cult. & Biotech. 17 PTC&B TM Binary Destination …

Webwere sterilized with 0.3% Sodium Hypochlorite and pre-germinated on 0.9% bactoagar plates at 30 C. Seven day old seedlings were transferred to sterilized sand and grown for two weeks without fertilizer. Plantlets were then transferred to liquid ½ Hoagland containing 25 M phosphate for another week before treating them either with GSE, WebNdeI for a single cut fragment, L7:1kb + DNA ladder, L1-L6: Digested samples, L2: Positive sample with true single cut. 1E: Plasmid isolation from pHGWFS7.0_Adh vector. phenom valuationWebThis intermediate entry vector was recombined with the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants were selected on Gamborg's B5 plates containing hygromycin (50 µg/liter). phenom volleyball

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Phgwfs7.0

miRNAs control HAM1 functions at the single-cell-layer level

WebMar 14, 2024 · Download. Get Updates Share This. Summary. Files. Reviews. Support. Code. OpenPFGW is software that is designed to perform PRP and primality tests on numbers of … WebAug 1, 2010 · GATEWAY-compatible destination vectors ( K arimi et al . 2002 ) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from the Department of Plant Systems Biology, VIB-Ghent University (Ghent, Belgium). The vector pAVA319 contained a translation leader (TL) and was obtained from ABRC.

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WebSep 14, 2011 · GATEWAY ™-compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), promoter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium). The LR reaction was … WebJul 23, 2010 · To assess the suitability of the NAPA promoter we made a translational fusion between the promoter fragment and the eGFP (encoding enhanced green fluorescent protein) and uidA [encoding β-glucuronidase (GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. …

WebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into … WebAug 1, 2010 · GATEWAY-compatible destination vectors (Karimi et al. 2002) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from …

WebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). Web(GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. GUS activity was observed in develop-ing seeds in several independent transformants, but was absent from other tissues and organs including pollen. GUS activity was reproducibly detected in embryos from late

WebCCATTAACAAATCTCCG-30; and a T-DNA insertion primer, LBb1.3, 5 0-ATTTTGCCGATTTCGGAAC-3 ). The zfp5 gis3, ... RNAi construct, whereas pHGWFS7 was used for the pGIS3:GUS construct. For all cloning constructs, targeted gene-speciﬁc fragments were ﬁrst PCR-ampliﬁed from cDNA phenom waste energy systemWebLOCUS pHGWFS7 13124 bp DNA circular 23-FEB-2024 DEFINITION . ACCESSION urn.local...1q-dnt3ece KEYWORDS Promoter analysis. phenom watch phoneWebMar 15, 2016 · The following binary T-DNA destination vectors were used: pMDC32, pMDC162 and pMDC204 (Curtis & Grossniklaus, 2003 ), and pHGWFS7,0 and pH2GW7,0 (Karimi et al ., 2002 ). The promoter region of the BDG gene was amplified as a 2596-bp fragment upstream of the BDG coding region using Gateway-compatible primers … phenom webmailWebApr 1, 2007 · Mature anthers were fixed in 4% paraformaldehyde and 0.25% glutaraldehyde under vacuum. Fixed samples were then dehydrated through a graded ethanol series followed by a t-butanol series, ... The binary vector pHGWFS7 was modified for the GUS gene under the control of a 2000 ... phenom volleyball watch listWeb(GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. GUS activity was observed in develop-ing … phenom watchlistWebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … phenom websiteWebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into Arabidopsis using Agrobacterium tumefaciens (strain EHA105)-mediated floral dip method (Clough and Bent 1998 ). phenom wealth financial