Ecto atpase

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WebPeter H. Cheung is an academic researcher from University of Nebraska Medical Center. The author has contributed to research in topic(s): Calcium ATPase & ATPase. The author has an hindex of 1, co-authored 1 publication(s) receiving 20 citation(s). WebMar 29, 2024 · The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. …

Combined biochemistry and histocytochemistry as a tool to …

WebApr 4, 2008 · In the facultative human pathogen Mycoplasma hominis, which belongs to the cell wall-less Mollicutes, the surface-localised substrate-binding domain OppA of the oligopeptide permease was characterised as the main ecto-ATPase. With the idea that extra-cellular ATP could only be provided by the infected host cells we analysed the ATP … WebJan 30, 2024 · Apyrase, purified from potato or recombinant organisms , is an ecto-ATPase which hydrolyzes a wide range of nucleotide (N) substrates including ATP and ADP, according to the general reaction NTP → NDP … edp cross id colombe

Exfoliation of membrane ecto-enzymes in the form of micro-vesicles

WebFeb 20, 2001 · We describe enhanced expression and enzymatic activity of ecto-ATPase and ecto-5′nucleotidase on CMV infected endothelial cells as compared to uninfected cells. These ectoenzymes play a major role in modulation of platelet activation and aggregation. Furthermore, adenosine has a modulatory effect upon inflammation. WebAug 8, 2012 · Blue native PAGE analysis for cell wall proteins from pea epicotyls demonstrated that cell wall-associated ecto-apyrase (ATPase) formed a large protein complex(es) ranging from 450 to 900 kDa; one of … WebIt remains to be determined whether CD39 or another ecto-nucleotidase is the predominant βγMeATP-sensitive ecto-ATPase expressed in 1321N1 human astrocytes. The … edp computing

Specificity of the ecto‐ATPase inhibitor ARL 67156 on human

954 - Gene ResultENTPD2 ectonucleoside triphosphate …

WebJan 1, 1995 · Ecto-ATPases are ubiquitous in eukaryotic cells. They hydrolyze extracellular nucleoside tri- and/or diphosphates, and, when isolated, they exhibit E-type ATPase activity, (that is, the activity is dependent on Ca 2+ or Mg 2+, and it is insensitive to specific inhibitors of P-type, F-type, and V-type ATPases; in addition, several nucleotide tri- and/or … WebATP was the best substrate for the yeast Mg(2+)-stimulated ecto-enzyme, but ADP, ITP, CTP, GTP and UTP were also hydrolyzed. A direct relationship between ecto-ATPase activity and adhesion to host cells was observed. In these assays, inhibition of enzyme activity resulted in decreased levels of yeast adhesion to epithelial cells. edp ct championship 2023WebMar 1, 2011 · There is loss of labelling for membrane pump Ca(2+)-ATPase activity on the nodal axolemma, together with loss of ecto-Ca-ATPase from the external aspect of the myelin sheath at sites of focal ... edp coated steel

"WebSep 10, 2002 · Ecto-ATPase (ecto-adenosine triphosphatase), a key enzyme of cardiac metabolism, is responsible for modulation of the concentration of extracellular nucleotides in the heart. We present methodology consisting of the combined use of biochemical and histocytochemical techniques to study its properties. Using samples from essentially the … " - Ecto atpase

Ecto atpase

The Multifaceted ATPase Inhibitory Factor 1 (IF1) in Energy …

WebDec 1, 2010 · ecto-F 1-ATPase activity or adenylate kinase (AK) activity which consumes ADP generated upon activation of ecto-F 1-ATPase, downregulates holo … WebCa2+ (Mg2+)-ATPase (ecto-ATPase) in rat hepatocytes was determined by several methods. 1) Two polyclonal antibodies specific for the ecto-ATPase were used to examine the distribution of the enzyme in frozen sec- tions of rat liver by immunofluorescence. Fluorescent staining was observed at the bile canalicular region of hepatocytes.

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WebDec 1, 2004 · The ecto-ATPase activity was increased at about five times (498.3 ± 27.6 nmol Pi h−1 mg−1) in the presence of 5 mM MgCl2, with values of V max and apparent K m for Mg-ATP2−corresponding to ... WebFirst, ecto-ATPase activity has been noted for α-sarcoglycan, indicating that α-sarcoglycan might function in controlling the ATP concentration at the surface of the muscle cell [117]. …

WebPPADS, suramin and reactive blue are ecto‐ATPase inhibitors and this property, combined with their antagonistic selectivity for receptor subtypes, can result in inhibition of, potentiation of, or lack of effect on agonist‐mediated PI responses. 1 Previous studies have shown that bovine pulmonary artery endothelium (CPAE) has P2Y and P2U purinoceptors, rat C6 … WebNational Center for Biotechnology Information

WebAug 22, 2003 · This study describes the possible role of Mg2+-dependent ecto-ATPase activity on the Trypanosoma cruzi–host cell interaction. Mg2+-dependent ecto-ATPase activity is observed on the cell body and flagellar membranes of the parasite and is about 20 times greater in trypomastigotes, as compared with epimastigotes. Suramin (a …

WebSep 20, 2011 · The K m value for ATP hydrolysis of curcumin-sensitive ecto-ATPase was similar to the value of NTPDase2, an isoform of ecto-nucleoside triphosphate diphosphohydrolase. These results suggest that curcumin is a potent inhibitor of ecto-ATPase and may affect extracellular ATP-dependent responses.

WebApr 26, 2007 · It should be noted, however, that the role of intracellular ATP as a source of intracellular energy was dominated by the discussion of possible ecto-ATPase functions. … edp dean smithThere are different types of ATPases, which can differ in function (ATP synthesis and/or hydrolysis), structure (F-, V- and A-ATPases contain rotary motors) and in the type of ions they transport. • Rotary ATPases • P-ATPases (E1E2-ATPases) are found in bacteria, fungi and in eukaryotic plasma membranes and organelles, and function to transport a variety of different ions across … edp cup spring/memorial day classic 2023WebLocalization of nucleotidases dependent on Ca 2+ was investigated cytochemically in axenically cultivated trophozoites of Entamoeba histolytica, strain HM-1:IMSS, with an electron microscope. Ca 2+-dependent ATPase (EC 3.6.1.3) activity was found on the plasma membrane and on the inner surface of the limiting membrane of a few … edp coated partsWebJul 1, 2006 · ATPase inhibitory factor 1 is a myokine inhibiting the hydrolytic activity of mitochondrial adenosine triphosphate synthase and ecto-F1-ATPase on the surface of many cells. edp cup spring 2022WebAug 1, 2024 · Importantly, ecto-F 1-ATPase interacts with major histocompatibility complex Class I molecules on the surface of nontumor cells and protects them from Vγ9/Vδ2 T lymphocyte recognition . In addition, ecto-F 1-ATPase activity contributes to cancer cell proliferation. This aspect is covered in the next section (IF1 in Cancer and Angiogenesis). edp coolWebPrior to the observations of Engelhardt, a fairly active cell surface ATPase had already been reported by Acs et al. (1954) in ascites tumour cells. A detailed analysis of the Ehrlich ascites tumour cell surface ecto-ATPase was later carried out … edp cup showcaseWebJan 13, 2016 · Chadwick and Frischauf (1997) cloned and mapped a human gene sharing considerable amino acid identity with the chicken muscle ecto-ATPase and the human lymphoid cell activation antigen CD39 ().Biochemical studies had indicated that the human CD39 protein is an ectoapyrase. Ectoapyrases show high levels of amino acid sequence … edp crime reports