WebDec 4, 2009 · Stock solutions of the substrates CTS, ECS, VTN, or FBN were diluted in medium M199, pH 7.2 (CTS, 1 μM; ECS, 32 μM; VTN, 123 nM; or FBN, 8 μg/ml), whereas FBS was used undiluted. Wells of a 96-well plate (Immulon 2 Flat Plate, Dynatech, Chantilly, VA, U.S.A.) were coated with 50 μl of either FBS or diluted substrate and incubated for at ... WebDec 16, 2024 · Thaw CTS-VTN (0.9 mg/mL) at room temperature (20°C–25°C) for 5–10 min, then place on ice. Note: CTS-VTN can be divided into usage-size aliquots in polypropylene tubes and stored at −60°C to −80°C. b. Room temperature storage and/or shaking might result in an appearance of light turbidity. This does not impact product …
GMP-grade neural progenitor derivation and differentiation from ...
Web2.7 Coating cell culture plates with VTN-N 1. Thaw one VTN-N vial at room temperature and aliquot in 60µl volume in sterile polypropylene tubes. Store aliquots at -80°C. 2. Dilute the appropriate volume of VTN-N stock in CTS™ DPBS-/-based on the number of plates required to a concentration of 0.5 µg/cm 2. 3. Add 6 ml of CTS™ DPBS WebCTS™ (Cell Therapy Systems) Vitronectin (VTN-N) Recombinant Human Protein provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When … how many bees are there in the world
Gibco CTS Vitronectin (VTN-N) Recombinant Human …
WebGibco™CTS Essential 8 Medium is the first globally available culture medium for human pluripotent stem cells (PSCs) forumlated with components not directly derived from … Web2. Cell Therapy Systems (CTS™) reagents are GMP-grade, serum-free, xeno-free, and animal origin-free formulations. Moreover, they have cell and gene therapy-specific … WebVitronectin (VTN-N) Gibco™ Vitronectin recombinant human protein provides a defined surface for feeder-free culture of PSCs. When used with Essential 8 Medium, VTN-N has … how many bees does it take to make 1 ounce