WebApr 2, 2024 · Chromium Single Cell 3’ v3.1 Gel Beads (10x Genomics, 2000164) were vortexed for 30 seconds and loaded into row 2 of the chip, along with Partitioning Oil (10x Genomics, 2000190) in row 3. A 10x Gasket (10x Genomics, 370017) was placed over the chip and attached to the Secondary Holder. WebThe Chromium Single Cell Feature Barcode technology offers a comprehensive, scalable approach to detect cell surface proteins along with the gene expression and immune …
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WebChromium Next GEM Single Cell Gel Bead Kit v3.1 (-80°C Freezer) Chromium Next GEM Chip G Single Cell Kit (Room Temp) Single Index Kit T Set A OR Dual Index Kit TT Set A (-20°C Freezer) Not included in the 10X kits: Cell Suspension (See following Instructions) 50% glycerol solution (Room Temp) Nuclease-free water (Room Temp) 10% Tween 20 … Webassets.ctfassets.net cscc oncology
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WebThe 10x Genomics Chromium Controlleris a single-cell profiling technology that enables the analysis of large cell numbers at a high capture efficiency (of up to 65%). The platform allows for high-throughput analysis in a variety of cell types as well as single-cell nuclei. WebApr 14, 2024 · Cells were finally resuspended in ice-cold phosphate buffer saline (PBS) and 0.5% BSA and loaded onto the 10X Genomics Chromium Controller. WashU Cohort 2 and healthy donor samples were loaded using 10X Genomics Chromium Next GEM Single-Cell 3′ GEM, Library and Gel Bead Kit v3.2. WebJun 13, 2024 · Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 (2 boxes in -20C Freezer and 1 box in -80C Freezer) Chromium Next GEM Chip G Single Cell Kit (Room Temp) Single Index Kit T Set A (-20C Freezer) Not included in the 10X kits: Cell Suspension (See following Instructions) 50% glycerol solution (Room Temp) dyslexia screening test adults