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Cholesterol uptake cell-based assay kit

WebThe LDL Uptake Assay Kit (Cell-Based) ab287862 (Preivously known as LDL Uptake Assay Kit (Cell-Based) K436) enables measurement of receptor-mediated LDL uptake … WebThe dysregulated homeostasis of cholesterol uptake, storage and efflux leads to the aggregation of foam cells under the arterial wall. At the same time, macrophages-secreted pro-inflammatory cytokines drive foam cells and vascular smooth muscle cells to migrate and proliferate, forming plaque lipid nuclei and fibrous caps [4–6].

Cholesterol Cell- Based Detection Assay Kit

WebMar 29, 2024 · e Representative images of Filipin-based assay to detect cholesterol in LNCaP cells treated with NTS, siMBTPS2 or siSREBP siRNA. Cells were treated for 48 h and kept in either medium supplemented ... WebProduct overview. Cholesterol/ Cholesteryl Ester Assay Kit ab65359 provides a simple method for sensitive quantification of total cholesterol, free cholesterol, and cholesteryl esters, by colorimetric or fluorometric … how many ribeyes in a side of beef https://myyardcard.com

Cholesterol Uptake Assay Kit - Sigma-Aldrich

WebCholesterol Assay Kit (Cell-Based) (ab133116) includes filipin III, fixative, and wash buffer in a ready to use format. It provides a simple fluorometric method to study mechanisms … WebEstablishment of a cell-based assay We have set up a cell-based screening method using fluorescent-labeled LDL uptake in HepG2 cells as an end point assay. We treated human liver HepG2 cells with a ‘gain-of-function’ mutant D374Y-PCSK9 protein at 1 µg/ml concentration (13.4 nM). As shown in WebApr 28, 2014 · For the luciferase assay, 20 μL of cell lysate was transferred to a test tube and assessed directly by means of a Lumat LB 9507 luminometer (Berthold Detection Systems, Pforzheim, Germany), using a luciferase assay kit from Promega. The protein content was quantified using a bicinchoninic acid (BCA) assay (PIERCE, Rockford, IL, … howdens camberley

EZcountTM MTT Cell Assay Kit - diagnocine.com

Category:Cell Based Assays Thermo Fisher Scientific

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Cholesterol uptake cell-based assay kit

Cholesterol Uptake Assay Kit - Sigma-Aldrich

WebHDL Uptake Assay Kit (Fluorometric) (ab204717) contains a fluorescently-labeled HDL that can be measured when taken up by cells. Unlabeled-HDL is included in the kit for … WebCell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs. 1 Cayman’s XTT cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular ...

Cholesterol uptake cell-based assay kit

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WebHeme oxygenase-1 (HO-1) plays a vital role in the catabolism of heme and yields equimolar amounts of biliverdin, carbon monoxide, and free iron. We report that macrophages engulfing either the low amount of heme-containing apoptotic thymocytes or the high amount of heme-containing eryptotic red blood cells (eRBCs) strongly upregulate HO-1. The … WebCell-based assays are used to analyze several living cell properties. These products may include cell growth and differentiation assays, apoptosis assays, and signal transduction assays. Cell Function Reagents and Kits (387) General Cell Based Assays (324) Cell Structure Reagents and Kits (243) Signal Transduction Reagents and Kits (149)

WebCayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently … Web2. About the Assay. The EZcount. TM. MTT Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the yellow colored water soluble Tetrazolium dye 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium ...

WebCell colony formation assay. The cell culture and p53 transfection was conducted as described in section “Real-time quantitative PCR (RT-qPCR).” The cells were digested with 0.25% trypsin solution and reseeded into 6-well plates at a density of 1.0×10 4 cells/well. After one week of the culture, the cells were treated with 70% cold ethanol ... WebCayman’s LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight™ 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the uptake of LDL at the single-cell level. In addition, multiplexing with other markers, such as LDLR expression, is possible to ...

WebMay 13, 2024 · The level of cholesterol uptake was determined by a cholesterol uptake cell-based assay kit (No. 600440, Cayman, Ann Arbor, MI, USA) according to the operation manual. 2.7. Statistical Analysis. Results are presented as means and standard deviations. Data analyses were performed with the SPSS 25.0 statistical software (IBM).

WebThe Cholesterol Uptake Assay Kit (Colorimetric/Fluorometric) LS-K162 is a fluorometric assay kit for the quantitative detection of Cholesterol uptake by adherent cells, screening of cholesterol uptake inhibitors, and evaluation of effect of drugs on cholesterol uptake. how many rib roasts in a cowWebAbout This Assay Cayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at the cellular level. The kit employs human LDL conjugated to DyLight™ 550 (product of Thermo Fisher Scientific Inc.) as a fluorescent probe for detection of LDL uptake into cultured cells. An LDL how many rib eye steaks per cowWebMar 5, 2015 · Cholesterol was measured following the protocol provided in the Cholesterol Uptake Cell-Based Assay Kit from Cayman Chemical Company (Ann Arbor, Michigan USA). Briefly, Caco-2 cells were incubated for 40 minutes in glucose free HBSS containing 20μg/ml NBD Cholesterol with or without 10ng/mL LPS, 100nM Aβ 1–40, 1μM Aβ 1–40, ... howdens calcutta marble worktopsWebProduct overview The LDL Uptake Assay Kit (Flow cytometry) (ab236208) employs human LDL conjugated to DyLight TM 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the uptake of LDL at the single-cell level. how many ribosomes are in a cellWebCayman’s Glucose Uptake Cell-based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. The kit employs 2-NBDG, a fluorescently labeled … how many ribosomes in a cellWebCholesterol Uptake Assay Kit - Information Assay Genies total cholesterol assay kit (Fluorometric) is based on the cellular uptake of a fluorescently tagged cholesterol … howdens canvey islandWebNov 18, 2014 · LDL Uptake Assay Kit (Cell-Based) (ab133127) provides a convenient tool for studying LDL uptake and regulation at the cellular level. This kit employs Human … howdens canopy cooker hood